Patterns of nuclear DNA degeneration over time — a case study in historic teeth samples
Identifieur interne : 000A96 ( Main/Exploration ); précédent : 000A95; suivant : 000A97Patterns of nuclear DNA degeneration over time — a case study in historic teeth samples
Auteurs : P. Wandeler ; S. Smith [Allemagne] ; P. A. Morin [Allemagne] ; R. A. Pettifor ; S. M. FunkSource :
- Molecular Ecology [ 0962-1083 ] ; 2003-04.
English descriptors
Abstract
The amount of nuclear DNA extracted from teeth of 279 individual red fox Vulpes vulpes collected over a period spanning the last three decades was determined by quantitative polymerase chain reaction (PCR). Although teeth were autoclaved during initial collection, 73.8% of extracts contained sufficient DNA concentration (> 5 pg/µL) suitable for reliable microsatellite genotyping but the quantity of nuclear DNA decayed significantly over time in a nonlinear pattern. The success of PCR amplification across four examined canine microsatellites over time was dependent on fragment size. By including data from two different tests for human contamination and from frequencies of allelic dropout and false alleles, the methodological constraints of population genetic studies using microsatellite loci amplified from historic DNA are discussed.
Url:
DOI: 10.1046/j.1365-294X.2003.01807.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000A01
- to stream Istex, to step Curation: 000A00
- to stream Istex, to step Checkpoint: 000696
- to stream Main, to step Merge: 000B30
- to stream Main, to step Curation: 000A96
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Patterns of nuclear DNA degeneration over time — a case study in historic teeth samples</title><author><name sortKey="Wandeler, P" sort="Wandeler, P" uniqKey="Wandeler P" first="P." last="Wandeler">P. Wandeler</name></author><author><name sortKey="Smith, S" sort="Smith, S" uniqKey="Smith S" first="S." last="Smith">S. Smith</name></author><author><name sortKey="Morin, P A" sort="Morin, P A" uniqKey="Morin P" first="P. A." last="Morin">P. A. Morin</name></author><author><name sortKey="Pettifor, R A" sort="Pettifor, R A" uniqKey="Pettifor R" first="R. A." last="Pettifor">R. A. Pettifor</name></author><author><name sortKey="Funk, S M" sort="Funk, S M" uniqKey="Funk S" first="S. M." last="Funk">S. M. Funk</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1F707C737DCC00C62D7CD2CBB02A200AB981DC10</idno><date when="2003" year="2003">2003</date><idno type="doi">10.1046/j.1365-294X.2003.01807.x</idno><idno type="url">https://api.istex.fr/document/1F707C737DCC00C62D7CD2CBB02A200AB981DC10/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000A01</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000A01</idno><idno type="wicri:Area/Istex/Curation">000A00</idno><idno type="wicri:Area/Istex/Checkpoint">000696</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000696</idno><idno type="wicri:doubleKey">0962-1083:2003:Wandeler P:patterns:of:nuclear</idno><idno type="wicri:Area/Main/Merge">000B30</idno><idno type="wicri:Area/Main/Curation">000A96</idno><idno type="wicri:Area/Main/Exploration">000A96</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Patterns of nuclear DNA degeneration over time — a case study in historic teeth samples</title><author><name sortKey="Wandeler, P" sort="Wandeler, P" uniqKey="Wandeler P" first="P." last="Wandeler">P. Wandeler</name><affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation></author><author><name sortKey="Smith, S" sort="Smith, S" uniqKey="Smith S" first="S." last="Smith">S. Smith</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Laboratory for Conservation Genetics, Max Planck Institute for Evolutionary Anthropology, Inselstrasse 22, Leipzig 04103</wicri:regionArea><placeName><region type="land" nuts="1">Saxe (Land)</region><region type="district" nuts="2">District de Leipzig</region><settlement type="city">Leipzig</settlement></placeName></affiliation></author><author><name sortKey="Morin, P A" sort="Morin, P A" uniqKey="Morin P" first="P. A." last="Morin">P. A. Morin</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Laboratory for Conservation Genetics, Max Planck Institute for Evolutionary Anthropology, Inselstrasse 22, Leipzig 04103</wicri:regionArea><placeName><region type="land" nuts="1">Saxe (Land)</region><region type="district" nuts="2">District de Leipzig</region><settlement type="city">Leipzig</settlement></placeName></affiliation></author><author><name sortKey="Pettifor, R A" sort="Pettifor, R A" uniqKey="Pettifor R" first="R. A." last="Pettifor">R. A. Pettifor</name><affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation></author><author><name sortKey="Funk, S M" sort="Funk, S M" uniqKey="Funk S" first="S. M." last="Funk">S. M. Funk</name><affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Ecology</title><idno type="ISSN">0962-1083</idno><idno type="eISSN">1365-294X</idno><imprint><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2003-04">2003-04</date><biblScope unit="volume">12</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="1087">1087</biblScope><biblScope unit="page" to="1093">1093</biblScope></imprint><idno type="ISSN">0962-1083</idno></series><idno type="istex">1F707C737DCC00C62D7CD2CBB02A200AB981DC10</idno><idno type="DOI">10.1046/j.1365-294X.2003.01807.x</idno><idno type="ArticleID">MEC1807</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0962-1083</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA degeneration</term><term>historic samples</term><term>microsatellite genotyping</term><term>qPCR</term><term>red fox</term><term>tooth</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The amount of nuclear DNA extracted from teeth of 279 individual red fox Vulpes vulpes collected over a period spanning the last three decades was determined by quantitative polymerase chain reaction (PCR). Although teeth were autoclaved during initial collection, 73.8% of extracts contained sufficient DNA concentration (> 5 pg/µL) suitable for reliable microsatellite genotyping but the quantity of nuclear DNA decayed significantly over time in a nonlinear pattern. The success of PCR amplification across four examined canine microsatellites over time was dependent on fragment size. By including data from two different tests for human contamination and from frequencies of allelic dropout and false alleles, the methodological constraints of population genetic studies using microsatellite loci amplified from historic DNA are discussed.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>District de Leipzig</li><li>Saxe (Land)</li></region><settlement><li>Leipzig</li></settlement></list><tree><noCountry><name sortKey="Funk, S M" sort="Funk, S M" uniqKey="Funk S" first="S. M." last="Funk">S. M. Funk</name><name sortKey="Pettifor, R A" sort="Pettifor, R A" uniqKey="Pettifor R" first="R. A." last="Pettifor">R. A. Pettifor</name><name sortKey="Wandeler, P" sort="Wandeler, P" uniqKey="Wandeler P" first="P." last="Wandeler">P. Wandeler</name></noCountry><country name="Allemagne"><region name="Saxe (Land)"><name sortKey="Smith, S" sort="Smith, S" uniqKey="Smith S" first="S." last="Smith">S. Smith</name></region><name sortKey="Morin, P A" sort="Morin, P A" uniqKey="Morin P" first="P. A." last="Morin">P. A. Morin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/RenardV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A96 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000A96 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= RenardV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:1F707C737DCC00C62D7CD2CBB02A200AB981DC10
|texte= Patterns of nuclear DNA degeneration over time — a case study in historic teeth samples
}}
| This area was generated with Dilib version V0.6.27. |  |